Volusia is now the third Central Florida county to make mask-wearing mandatory for students and staff in public schools.
And Lake and Seminole County school boards are taking up COVID-19 issues at special meetings Thursday morning.
Last Friday, a Leon County circuit judge overruled Gov. Ron DeSantis's ban on school mask mandates, which aim to slow the rampant spread of COVID-19.
Since then, the school boards in Brevard and Volusia counties have imposed temporary mask requirements without a parental opt-out.
Orange County was already defying the governor's order.
The Volusia County board approved its mask mandate Tuesday night. It takes effect next Tuesday in all school district buildings and buses and will remain in effect until Oct. 15.
The Seminole County School Board is meeting at 8:30 a.m. Thursday in its boardroom in Sanford.
The Lake County meeting starts an hour later at the county administration building in Tavares.